It has already been performed by popular creators, like Charli D’Amelio: with THANK YOU SO MUCH□□□□□□Ĭreators and video of the quick-paced dance took an unexpected, but amusing turn, leading to over 982,000 likes and 4.7 million views. One year ago 6ix9ine dropped his first single since released from prison Gooba,it went top 3 on the hot 100 and is one of the best selling song and most. The dance began rising in popularity in June. ![]() DANCE!!! tag us #fyp GOOBA – OUR NEW DANCE!!! #fyp #foryou Leave it to the double thick thighs, twin sistersĭrop it down and wobble, wobble up, mami booted upĬreators and both shared the dance on their respective accounts as far back as May 2020. The dance was created by TikTokers and At the time of publication, there are over 744,000 videos linked to the sound, many of which feature the Gooba Challenge being performed. Two weeks after ‘GOOBA’ was released, 6ix9ine shared an Instagram post in which he promised, a kitten in each hand, that his next single and video would break the internet. Gooba, Trollz which was 1 on the top 100 billboards and YaYa. ![]() The 'GOOBA' rapper is refusing to leave Florida - even when his safety is on the line. ChadForever Ft Gooba an Danga(Feedback)dance video from gooba. His 1st 3 songs were made from the house he was renting due to being on house arrest. Rapper Tekashi 6ix9ine is recovering from the beating carried out by three or four thugs in the bathroom of a South Florida gym Tuesday night. Jump To 6ix9ine gooba first live performance since being released 2020 preview 1 Video. He made Gooba right after he got out and released it about a month later. ![]() The #GoobaChallenge on TikTok is an incredibly fast dance routine performed to Tekashi 6ix9ine’s “Gooba.” The dance is characterized by throwing it back, twerking, and crossing arm motions. AthElite Dance, Shiremanstown, Pennsylvania. Download 6ix9ine gooba free mobile Porn, XXX Videos and many more sex. How does 6IX9INE make GOOBA music video in jail He didn’t.
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The ratio of absorbencies of the DENRA and the control antigen wells determines whether the result is positive or negative.(Package insert: InBiOS DENV Detect IgM CAPTURE ELISA. Acid stop is added, and absorbance at 450 nm is read. After a second incubation and washing step, the wells are incubated with tetramethylbenzidine (TMB) substrate. After incubation and washing, the wells are treated with a DEN-specific monoclonal antibody labeled with horseradish peroxidase (HRP). ![]() This incubation is followed by incubation with dengue-derived recombinant antigens (DENRA) and normal cell antigen separately. In this enzyme-linked immunosorbent assay (ELISA), samples and controls are diluted in sample dilution buffer and incubated in microtiter wells coated with antihuman IgM antibodies. Note that the InBios DV IgG EIA shows significant cross-reactivity with antibodies to West Nile virus and St. Louis encephalitis virus, and California (LaCrosse) virus Cross-reactivity of the InBios DV IgG EIA with antibodies to West Nile virus, St. Louis encephalitis virus (n=9), and California (LaCrosse) virus (n=9) were also tested by the InBios DV IgG EIA and the data are summarized below in Table 3. ![]() Specificity: 100% (164/164) 95% CI: 97.2%-100%Īn additional 42 serum samples positive for IgG-class antibodies to West Nile virus (n=24), St. Comparison of the InBios and Focus (Quest) Diagnostics DV IgG EIA Comparison of the InBios and Focus (Quest) Diagnostics DV IgM EIA The results were compared and the data summarized in Tables 1 and 2. The DV NS1 antigen can be detected by ordering DNSAG / Dengue Virus NS1 Antigen, Serum.Ī total of 200 prospective serum samples submitted for dengue virus (DV) IgM and IgG testing by the Focus Diagnostics DV IgM and IgG enzyme immunoassays (EIA) were also tested by the InBios IgM and IgG DV assays. NS1 antigenemia is detectable within 24 hours of infection and up to 9 days following symptom onset. As an adjunct to serologic testing, identification of early DV infection may be made by detection of the DV nonstructural protein 1 (NS1) antigen. Seroconversion occurs approximately 3 to 7 days following exposure, and therefore, testing of acute and convalescent sera may be necessary to make the diagnosis. However, children and young adults remain at increased risk for progression to dengue hemorrhagic fever and dengue shock syndrome, particularly during repeat infection with a new DV serotype.ĭetection of dengue-specific IgM and IgG-class antibodies remains the most commonly utilized diagnostic method. Fever defervescence signals disease resolution in most individuals. Symptomatic patients become acutely febrile and present with severe musculoskeletal pain, headache, retro-orbital pain, and a transient macular rash, most often observed in children. DV poses a significant worldwide public health threat with approximately 2.5 to 3 billion people residing in DV endemic areas, among whom 100 to 200 million individuals will be infected, and approximately 30,000 patients will succumb to the disease, annually.įollowing dengue infection, the incubation period varies from 3 to 7 days, and while some infections remain asymptomatic, the majority of individuals will develop classic dengue fever. It is primarily transmitted by the Aedes aegypti mosquito, which is found throughout the tropical and subtropical regions of over 100 countries. "I am not alone": explore 1920s Norway.Dengue virus (DV) is a globally distributed flavivirus with 4 distinct serotypes (DV-1, -2, -3, -4).And what begins as a quest for a missing woman becomes a journey into a painful past. Together with Edward and Lissie, you must explore a forgotten coastal community nestled amongst the dark fjords and towering mountains of rural Norway, and unearth the terrible secrets that lie beneath the picturesque surface. ![]() But you're never alone: every step of the way, Edward is accompanied by Lissie: a lively and enigmatic young woman with a mind of her own. You play Edward Charles Harden, an American traveller - and increasingly unreliable narrator - who's come to Norway to find his missing sister. Nominated for Best Art at the Nordic Game Awards.įrom the studio that brought you Dreamfall Chapters, and the creative team behind The Longest Journey and The Secret World, comes a first-person psychological mystery set in 1920s Norway. Winner of Game of the Year and Best Audio awards at Spillprisen (the Norwegian Game Awards). Draugen is a single-player, first-person Fjord Noir tale of suspense, mystery and deception, set in 1920s Norway. Here is a collection of some of the best and most popular animated wallpapers we’ve found on Windows 10. Check your new Windows 10 live wallpaper Press Start + D to return to your desktop and view your brand new animated wallpaper in Windows 10. The Best Animated Wallpapers for Windows 10 Thousands of new 4k videos every day Completely Free to Use High-quality HD videos and clips from Pexels. Ever dreamed of having the entirety of Shrek as your background? Now you can. Download and use 14,312+ Live wallpaper 4k stock videos for free. PUSH Video Wallpaper is another paid solution that includes a variety of animated backgrounds, but it gives you the ability to use feature-length movies as your wallpaper. ![]() It allows you to download dynamic wallpapers from its collection of thousands of wallpapers organized under categories like Cats, Nature, River, Search, Sun, etc. It is used to install numerous wallpapers, both free and paid. Get creative with our collection of stunning wallpaper templates you can customize with over a million images, icons, and illustrations from the Canva. Desktop Live Wallpapers is one of the best apps offering animated wallpapers for your desktop. This application is $4 and has hundreds of user-created, high-quality backgrounds.ĭesktop Live Wallpapers is another app available for download for free through the Microsoft Store. One of the best places to find animated wallpapers is through the Wallpaper Engine on Steam. ![]() Next, in the main VLC window, go to the Crop settings and choose the correct ratio for the display you are using. 3 Click Get to download Lively Wallpaper. Click the tile for Lively Wallpaper by rocksdanister. ![]() You can find the search field at the very top the screen. 1 2 Search for Lively Wallpaper in the search bar. These tools will let you set animated or live wallpapers on your machine. Finally, switch back to Simple settings, then under Video make sure your Output drop-down in the Display section is set to Automatic. Lively Wallpaper is a free and open-source software for animated desktop wallpapers. To do this, open a File Explorer window and head to the following folder: C:WindowsWebWallpaper. You will also need a registered version of Windows 10 you can’t get away with the free version because it locks down the personalization option.ĭownload Rainmeter, Desktop Live Wallpapers, or another tool and follow the setup instructions. Since Windows 10 gives you access to all the stock wallpapers, you can open this wallpapers folder and set the original wallpaper as your desktop background. There are a variety of programs that allow you to set live or animated wallpapers on Windows 10, and you may need one or more of these programs to use some of the entries on this list. This cookie is used to track how many times users see a particular advert which helps in measuring the success of the campaign and calculate the revenue generated by the campaign. This cookie is set by Google and stored under the name. The data collected including the number visitors, the source where they have come from, and the pages viisted in an anonymous form. The cookie is used to store information of how visitors use a website and helps in creating an analytics report of how the wbsite is doing. This cookie is installed by Google Analytics. The cookies store information anonymously and assign a randomly generated number to identify unique visitors. The cookie is used to calculate visitor, session, campaign data and keep track of site usage for the site's analytics report. ![]() ![]() The cookies is used to gather information regarding visitor activity on Issuu products. This cookie is used by Issuu analytic system. These cookies help provide information on metrics the number of visitors, bounce rate, traffic source, etc. It does not correspond to any user ID in the web application and does not store any personally identifiable information.Īnalytical cookies are used to understand how visitors interact with the website. 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These cookies ensure basic functionalities and security features of the website, anonymously. Necessary cookies are absolutely essential for the website to function properly. Fry the eggs sunny side up and season the eggs with salt and pepperġ) Place all the corned beef hash into a serving bowl. Allow the corn beef to cook for about 3 minutes.Ĥ) Add onions and peppers and cook for another 4 minutes.ĥ) Next add the potatoes and cook for another 3 minutes.Ħ) Remove the mixture from the heat and fold in diced Swiss cheese.ħ) Season the hash with salt and pepper and set aside.Ĩ) In a separate pan over medium heat with a small amount of oil, add eggs. The potatoes should be just cooked through.Ģ) Strain potatoes into a colander and allow the potatoes to cool.ģ) In a large sauté pan over medium-high heat, add diced corned beef. Allow the potatoes to cook for about 12 minutes. Cook meat for about 1 hour.Ħ) Pull brisket from oven and cover with aluminum foil.ħ) Place back in oven for approximately 1 more hour or until brisket is tender.Ĩ) Allow brisket to rest for about 20 minutes.ġ) In a large mixing bowl add the ketchup, mayonnaise, sweet pickle relish, and sriracha and incorporate.Ģ) Season with salt and pepper and set aside.ġ) Place fingerling potatoes into a pot of seasoned water and bring to a boil. ![]() Allow to cool down you should be able to touch it with your hands.Ĥ) In a large mixing bowl, combine coriander with black pepper, salt, and smoked paprika and rub generously on the meat.ĥ) Pre-heat oven to 300. ![]() Cook for about 4 to 5 hours at hard simmer.ģ) Remove beef from the pot and place on a large baking sheet. 1) In a large pot add beef brisket and cover the brisket with water.Ģ) Add half the coriander, half the black pepper, and all the bay leaves and garlic cloves. 6) Once you are logged in, click on My Games on the left side of the screen. 4) Log into your MacGameStore account by clicking on the top right corner. After hours and numerous attempts with site support, which is only web form, I have only received automated messages. Terry walters machine shop roanoke va, Suzuki peewee 50, Rod gellatly. Double-click on it to run the MacGameStore App. Effectively, Macgamestore took my money and have not provided the product purchased. Deichkind arbeit nervt album zippy, Shop tv in the philippines. Kichiku megane r cg zip, Mw3 save game and player profile, En otra vida emmanuel. I do not feel this is something an official distributor should do, and hopefully they do something about this as we are all humans as they are and surely they themselves have made purchases in their life that may have been either due error or them changing their mind when they haven't used or opened the product. Best rpg games ps vita, Sanuk coupon code september 2015, World 7 tower ds, 141. You really don't have an option there and it could quite be a hassle to get a refund since most retailers have the no refund policy upon revealing the key, however they make it so you clearly have to reveal it yourself. How long does it hurt after vasectomy, Mac game store helper, The grimoire. There should be no need to re-buy the mac versions of the same games, though you will need to download the mac versions of the game to your new computer. I've noticed they are coming with good deals recently which is comparable to other official retailers like Humble/Fanatical, hope they keep that up, one of the reasons why I ended up making more purchases even in the last 2 months! One little qualm though and can be quite worrisome is, they tend to reveal the key right away after the purchase whereas many others don't in case you make a mistake and purchase something in error. Buying back into game mvm, The inbetweeners season 1 episode 7 full episode. Made a few purchases throughout the few years and haven't had any issues. Find the game you want to install and click on 'Install' on the right side. If you don't have Steam installed on your Mac, the MacGameStore App will download Steam and install it for you.ĥ) On launch of Steam and this is your first time using Steam, it will ask you to login or create a new account.Ħ) Once you are logged into Steam, click on + ADD A GAME. in the lower left side of the Steam screen.ħ) Then select Activate a Product on Steam.Ĩ) Enter the Steam code by pasting the key you copied in Step 3.No issues but slight worrisome regarding refund policy. Double-click on it to run the MacGameStore App. The app utilizes the cloud so games are downloaded extremely quickly and allows customers to store their games in the cloud. This new app provides game screenshots, game trailers, community reviews, and game ratings. Click on Copy to copy it to your clipboard.Ĥ) Click the Play on Steam button to launch Steam. MacGameStore has an extensive library of Mac games with new games being added daily. Bonus points apply above and beyond what you would normally get for the purchase If the bonus has an 'x' (like 'x2' or 'x3'), points you would normally get will be multipled (a 9.99 game may give you +10 points, but a bonus of 'x2. On the right side click on Play On Steam.ģ) The screen will show you the Steam code required to activate a game on Steam. Normally when you purchase a game, you get points based off the price of the game and your membership tier. Build-a-bundle from 2 games for 1 to all for 3. If you have purchased a game that requires Steam, please following these instructions to activate the game.ġ) Launch the MacGameStore App and make sure you are logged into your account (top right corner).Ģ) Go to MY GAMES → PURCHASES and find the game. in order to trade DRM-free stuff, must have kept it unrevealed. Two can challenge each other to a blast around the track, or. ![]() Its a wild race, made even wilder with all the bombs and action of Bomberman. Akumajou Dracula X: Gekka no Yasoukyoku (v1. It sure isnt like your typical racer - Bomberman Fantasy Race has players riding on big loping kangaroos and giant blue rabbitty-dinos as they hop and dash to the finish line with long, bounding gaits.Ai to Yuujou no Neko Monogatari.: Jingle Cats: Love Para Daisakusen no Maki (Japan) Download Bomberman Fantasy Race (USA) (147M) Note: This game is a PSX2PSP eBoot and is meant to be played on a Sony PSP. Bomberman Kart is a kart-racing game starring Bomberman and friends, this time replacing the oddball mounts of Bomberman Fantasy Race with traditional.Advanced Dungeons & Dragons: Iron & Blood: Warriors of Ravenloft (USA).Adidas Power Soccer International ’97 (Europe).Action Replay 2 Version 2.30 (Bonus PSone Codes!).A Ressha de Ikou 4: Evolution Global (Japan). ![]()
Jeremy Lynn (born June 12, 1963), better known by the ring name Jerry Lynn, is an American retired professional wrestler currently signed with All Elite Wrestling as a producer and coach.Funeral Home: Kingston New York Area Obituariesĭonald "Danny" Richard Simmons, Sr, of Kingston, NY ended his journey on Earth December 17, 2017, at the home of his beloved daughter Simmone. He has worked for promotions such as World Championship Wrestling (WCW), Extreme Championship Wrestling (ECW), the World Wrestling Federation (WWF), NWA Total Nonstop Action (NWA TNA), and Ring of Honor (ROH). Lynn is a two time world heavyweight champion, having held the ECW World Heavyweight Championship once and the ROH World Championship once. Other championships held by Lynn in his career include the GWF Light Heavyweight Championship ( once), WWF Light Heavyweight Championship ( once), the TNA X Division Championship ( twice), the NWA World Tag Team Championship ( twice) and the WWA International Cruiserweight Championship (once). Professional wrestling career Early career (1988–1991) 2 Professional wrestling style and legacy.1.13 All Elite Wrestling (2019–present).1.5.2 Feuding with Rob Van Dam and World Heavyweight Champion (1999–2001).1.5 Extreme Championship Wrestling (1997–2001).Lynn retired as an active wrestler on March 23, 2013, exactly 25 years after his career began. Jeri lynn quarantino kingston ny professional# Lynn started wrestling in March 1988 in various independent promotions in the Minnesota and Michigan areas, one being Pro Wrestling America, where he won both their Heavyweight and Light Heavyweight championships. Lynn first joined the World Wrestling Federation in May 1989 as enhancement talent, losing to Rick Martel, and teaming with Ray Brown in a loss to Big Boss Man and Akeem later that night. In 1989, he began wrestling for Verne Gagne's American Wrestling Association, where by the end of 1990, he became the last challenger for the AWA World Heavyweight Championship, held by Larry Zbyszko, before Zbyszko left for World Championship Wrestling. In 1990, he made his first overseas trip to Japan, wrestling for Universal Lucha Libre. In January 1991, he wrestled for the United States Wrestling Association in Memphis and took part in a tournament for the USWA Southern Heavyweight Championship he lost to Tony Anthony in the first round. In June 1991, Lynn wrestled for the Global Wrestling Federation (GWF) based in Dallas, Texas. ![]() While in GWF, Lynn captured the GWF Light Heavyweight Championship. Lynn also engaged in a two-year feud with The Lightning Kid which gave both men considerable exposure in North America. In February 1992, he had a brief stop with Smoky Mountain Wrestling in Knoxville. In 1992, Lynn would take a role as an unnamed high school senior and as a stuntman in the movie Crossing the Bridge to prepare for the role, he did an angle with the Lightning Kid, where Kid cut Lynn's hair and sold it in small bags at $4 a bag, making a total $80. In December 1993, he returned to Japan for a tour with Michinoku Pro Wrestling. In July 1994, he went to Mexico under the masked alter-ego, Sultan Gargola for Consejo Mundial de Lucha Libre. In July 1995, Lynn returned to Michinoku Pro under the masked alter-ego Golgotha Cross, a villain from the Tiger Mask manga. Less than a month later, he would lose his mask to Gran Naniwa. World Championship Wrestling (1995–1997) In April 1995, Lynn returned to the WWF in a singles loss to Rad Radford and teamed up with Lenny Lane in a loss to The Heavenly Bodies the next night. In September 1995, Lynn joined World Championship Wrestling, alternating between wrestling under his real name and under the masked alter-ego Mr. He joined WCW's cruiserweight division, where he competed against the Mexican luchadores and a variety of wrestlers from around the world such as Chris Benoit, Dean Malenko, Eddie Guerrero, Sabu, Chris Jericho and Alex Wright. Lynn suffered a broken arm on December 18, 1995, in a match against Dean Malenko that would air one week later on Nitro on Christmas night. went to New Japan Pro-Wrestling (NJPW), representing WCW for their annual Best of the Super Juniors league he placed fifth on Block A with two confirmed points. ![]() One of his last major matches took place at the Clash of the Champions XXXIV, where he suffered a six-man tag team loss while teaming with Konnan and La Parka against Chris Jericho, Super Calo and Chavo Guerrero Jr. While he was injured, Eric Bischoff fired him from WCW in July 1997. ![]() Jeri lynn quarantino kingston ny professional#.Jeri lynn quarantino kingston ny movie#. ![]() Finally, the first column (1's column) has three beads counted. The second column (10's column) has a Heavenly bead counted for 50, and three Earthly beads counted for 30 giving it a total of 80. The third column (100's column) has two beads counted for 200. For example, the picture of the abacus is equal to "283" with nine beads moved towards the reckoning bar. To count to higher numbers, move further left, depending on how high you want to count. If you wanted the total to be seven, move two earthly beads up to the reckoning bar (5 + 2 = 7). At the same time, move all Earthly beads down. Because the modern abacus only has four Earthly beads, if you want to count to five, you must move the heavenly bead down to the reckoning bar using your index finger. Slide three more beads up make the abacus value four (3 + 1 = 4). One bead touching the reckoning bar makes the abacus equal 1. To count on the abacus start on the far right side of the abacus, and slide one earthly bead up to the reckoning bar using your thumb. If you have a reset button, press it to reset the beads. To use the abacus, lay it on a flat surface and set it to zero by making sure no beads are touching the reckoning bar. Going from right-to-left, the beads values increase to the 10's place, 100's place, 1,000's place, etc. The beads values start in the far right 1's column, which are beads valued between 1 and 9. These markers can also mark your first position if you do not want to start counting from the far right. The separation dots and off-colored beads vary depending on the abacus, but are always used to separate numbers into sets of three. When moving a bead towards the middle bar (reckoning bar), it's considered counted and when no bead is touching the reckoning bar that column is equal to zero. The lower beads are called "Earthly beads" and are each worth one in the first column. The upper beads are called the "Heavenly beads" and are worth five in the first column. We are using the modern abacus for examples on this page. The modern abacus, Japanese abacus, or soroban has four beads at the bottom and one bead at the top. For example, the classical abacus or Chinese abacus has five beads on the bottom and two beads at the top. How to use the abacusīefore learning to use the abacus, realize there are different types of abacus'. By learning to solve problems in new ways, you can come up with better and often easier solutions to all sorts of problems. Learning to use the abacus helps teach you a new way of counting and how to add and subtract using complementary numbers. Yes, even today in certain parts of the world the abacus is still used as a primary counting device or as a backup to more modern counting devices. Before the abacus, the only methods people had to use for their mathematical calculations were their fingers and toes, or stones in the dirt. Why was the abacus used?īefore computers, calculators, or even arithmetic using paper and pencil, the abacus was the most advanced device for crunching numbers. The abacus was also used in other early civilizations, including the Chinese, Egyptian, Greek, Persian, and Roman civilizations. ![]() There is evidence of the abacus used in Mesopotamia going back as early as 2700 B.C., for use with their sexagesimal numbering system. It is still unknown who invented and built the first abacus and when it was built. Using complementary numbers to subtract on the abacus.Using complementary numbers to add on the abacus.Why should I learn or teach the abacus?. ![]() Now, even micro-businesses and solo-preneurs can turn any phone or group of mobile phones into a professional, unified phone system with a virtual assistant (press 1 for sales, press 2 for support), multiple extensions, toll-free numbers, and so much more. By providing affordable phone solutions that formerly were only available to big business enterprises, VoxDirect is giving small businesses a big voice. While work habits have continued to shift in the marketplace, the utilization of cloud-based communication tools and work-from-home solutions have paved the way for a new workforce to emerge. It’s also the foundation behind our community of Independent Certified Consultants that will help us reach the millions of small business owners today.” That is why we sincerely believe our brand promise of ‘Making life better for many’ will drive innovation and help us continue to deliver great value to the small business community. And second, they have created a unique compensation model for their work-from-home consultants that allows ordinary people to earn extraordinary income by helping the small business community.Įxecutive Vice President Staci Wallace explains the Voxox and VoxDirect strategies by saying, “We create easy-to-use communication and marketing tools that help small business owners automate the often laborious and time-consuming activities they don’t like to do so they can get back to doing what they really love. First, they are driving down the cost of must-have communication tools. Voxox has solved two major needs in the small business marketplace. More than just an ideology, Voxox has made it a driving core value in both customer support and how they reward their communication consultants that represent their VoxDirect brand. What makes this company so intriguing is its “people first” culture. We believe in small business owners as the catalyst for economic growth and have made it our mission to innovate new communication solutions with them and their customers in mind,” says Executive Chairman and Co-founder Bryan Hertz. Why Small Businesses? “We strongly believe that small businesses are the lifeblood of the American economy. ![]() We want to use magnification in the microscope objective range, ×5 and above, so our ability to use conventional macro lenses is limited. This subject is small so magnifications above 1:1 will be needed to reveal its detail, but it is significantly thick and three dimensional so we cannot simply put it on a slide, squash it with a coverslip and examine it with the microscope. Let us suppose we wish to take a highly detailed picture of the head and eyes of a dead fly. This site is primarily for those interested in all aspects of the microscope and microscopy rather than the world of macrophotography, but increasingly these two interests are overlapping and coalescing as macrophotographers tackle smaller subjects and higher magnifications. In principle any semi-transparent structures which lie above or below other similar structures, can create headaches in stacking.īefore moving to consideration of the choice of stacking software, it is worthwhile to mention stacking in high magnification macrophotography. There is no substitute for retouching in this case, and Zerene Stacker’s ability to retouch the output from single frames from the stack is a powerful help. The result can be loss of detail and semi-transparent hairs instead of solid ones. A notorious example is fine overlapping hairs, when the software finds it difficult to decide what is above and what below. ![]() Certain kinds of structure cause problems to all stacking software.Stacking with objectives above ×40 requires a precise fine focus and a steady hand. Start with a subject that is suitable for a moderate magnification objective, ×10 – ×25 is ideal.Best to minimise them by having a clean sensor to start with. Dust spots on the camera sensor create irritating trails during the operation of the stacking software, which appear on the final output and can give some tedious and tricky cleanup tasks in post processing if they are numerous. Make sure your camera sensor is as clean as possible before you start.Failure to allow enough overlap causes banding to appear in the stacked output. ![]() This is important for the stacking software to work properly. Allow plenty of overlap between steps the idea is to move smoothly though the depth of the subject just as though you were focusing normally.If you have good parfocality between your visual eyepieces and your camera, and your trinocular head permits simultaneous viewing and photography, then it is possible to judge steps by eye, otherwise use the tick marks on your fine focus and be careful not to loose concentration and forget which direction you are turning the focus wheel!.Check visually that the size of focus step you have chosen is appropriate to the subject and magnification.Stacking works best with a series of approximately equal focus steps between top and bottom settings.bottom → top or top → bottom, not randomly. Stacking software needs images to be acquired in a logical sequence, i.e.Note the settings of the fine focus at the top and bottom focal plane of the subject. Make sure your fine focus operates smoothly.Start with a nice simple prepared slide, choosing a subject with significant depth. Practise your stacking technique on subjects that don’t move! Live subjects like water fleas and mosquito larvae make attractive subjects for stacking, but you need to learn how to confine or anaesthetise them first.Stacking inevitably has its limitations, and there are a few general rules that will help you to make better stacked images. What if we took a new picture each time we made an adjustment to the fine focus, and then combined each of these ‘slices’ together to reconstruct the full depth of the subject? In fact we can do exactly this and use software to select the sharply-focused parts of each slice and combine them into a single composite image showing greatly extended apparent depth of field. That way, we get a much better impression of the 3D structure we are seeing. To appreciate them, we use the fine-focus control to bring various parts into sharp focus in sequence. ![]() Many of the subjects that we enjoy examining through our microscope have significant depth. Stacking is the process of combining a series of digital images of the same subject area, taken at different focal planes, into a single image with great depth of field, using a computer program. Home | Resources | Stacking & stitching | Stacking Stacking Enhancing depth of field by combining image stacks Introduction Bulletin of the Quekett Microscopical Club. |